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神经科学

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    现刊
    Preparation of Teased Nerve Fibers from Rat Sciatic Nerve
    利用大鼠坐骨神经分离制备单根神经纤维
    作者:Jinkun Wen, Lixia Li, Dandan Tan and Jiasong Guo日期:10/05/2017,浏览量:68,Q&A: 0
    Compared to tissue sectioning techniques, the technique of teasing single nerve fibers provides a better way to understand the structures of myelin sheaths and axons of the peripheral myelinated nerves. This protocol describes a method for ...
    Preparation of Teased Nerve Fibers from Rat Sciatic Nerve
    利用大鼠坐骨神经分离制备单根神经纤维
    作者:Jinkun Wen, Lixia Li, Dandan Tan and Jiasong Guo日期:10/05/2017,浏览量:68,Q&A: 0
    [Abstract] Compared to tissue sectioning techniques, the technique of teasing single nerve fibers provides a better way to understand the structures of myelin sheaths and axons of the peripheral myelinated nerves. This protocol describes a method for preparation of teased single nerve fibers from rat sciatic nerve. In this protocol, fixed nerves are teased ...
    Bioluminescence Monitoring of Neuronal Activity in Freely Moving Zebrafish Larvae
    自由移动的斑马鱼幼鱼中神经元活性的生物发光监测
    作者:Steven Knafo, Andrew Prendergast, Olivier Thouvenin, Sophie Nunes Figueiredo and Claire Wyart日期:09/20/2017,浏览量:96,Q&A: 0
    [Abstract] The proof of concept for bioluminescence monitoring of neural activity in zebrafish with the genetically encoded calcium indicator GFP-aequorin has been previously described (Naumann et al., 2010) but challenges remain. First, bioluminescence signals originating from a single muscle fiber can constitute a major pitfall. Second, ...
    Stereotaxic Adeno-associated Virus Injection and Cannula Implantation in the Dorsal Raphe Nucleus of Mice
    在小鼠中缝背核立体定向注射腺相关病毒和埋植套管
    作者:Patrícia A. Correia, Sara Matias and Zachary F. Mainen日期:09/20/2017,浏览量:92,Q&A: 0
    [Abstract] Optogenetic methods are now widespread in neuroscience research. Here we present a detailed surgical procedure to inject adeno-associated viruses and implant optic fiber cannulas in the dorsal raphe nucleus (DRN) of living mice. Combined with transgenic mouse lines, this protocol allows specific targeting of serotonin-producing neurons in the ...
    Preparation of Primary Cultures of Embryonic Rat Hippocampal and Cerebrocortical Neurons
    胚胎大鼠海马和脑皮质神经元原代培养物的制备
    [Abstract] This protocol aims at standardizing the procedure to obtain primary cultures of hippocampal and cerebrocortical neurons for in vitro experiments. Cultures should be prepared from cells isolated during embryonic development when neuronal precursor cells are not yet fully differentiated. This helps increasing the quality and quantity of ...
    Arginine-rich Peptides Can Actively Mediate Liquid-liquid Phase Separation
    富含精氨酸的肽可以主动介导液-液相分离
    作者:Steven Boeynaems, Mathias De Decker, Peter Tompa and Ludo Van Den Bosch日期:09/05/2017,浏览量:246,Q&A: 0
    [Abstract] Studying liquid-liquid phase separation (LLPS) of proteins provides key insights into the biogenesis of membraneless organelles and pathological protein aggregation in disease. We have established a protocol for inducing the phase separation of arginine-rich peptides, which allows for studying their molecular determinants and dynamics (Boeynaems ...
    Isolation of Rodent Brain Vessels
    啮齿动物脑血管分离
    作者:Cristina Carvalho and Paula I. Moreira日期:09/05/2017,浏览量:190,Q&A: 0
    [Abstract] The prevalence of neurodegenerative diseases is increasing worldwide. Cerebrovascular disorders and/or conditions known to affect brain vasculature, such as diabetes, are well-known risk factors for neurodegenerative diseases. Thus, the evaluation of the brain vasculature is of great importance to better understand the mechanisms underlying brain ...
    Predator Odor-induced Freezing Test for Mice
    捕食者气味诱导的小鼠僵住试验
    作者:Shintaro Otsuka日期:09/05/2017,浏览量:167,Q&A: 0
    [Abstract] The innate fear response is an emotional response that does not require any previously acquired conditioning. One of the standard methods to analyze the innate fear response is a 2,4,5-trimethylthiazoline (TMT)-induced freezing test. TMT is an odor originally isolated from anal secretion of the red fox. Acute TMT exposure has been shown to induce ...
    FM1-43 Photoconversion and Electron Microscopy Analysis at the Drosophila Neuromuscular Junction
    果蝇神经肌肉接头处FM1-43光转换和电子显微镜分析
    作者:Nadezhda S. Sabeva and Maria Bykhovskaia日期:09/05/2017,浏览量:258,Q&A: 0
    [Abstract] We developed a protocol for photoconversion of endocytic marker FM1-43 followed by electron microscopy analysis of synaptic boutons at the Drosophila neuromuscular junction. This protocol allows detection of stained synaptic vesicle even when release rates are very low, such as during the spontaneous release mode. The preparations are ...
    An ex vivo Perifusion Method for Quantitative Determination of Neuropeptide Release from Mouse Hypothalamic Explants
    用于定量测定小鼠下丘脑外植体神经肽释放的离体灌流方法
    作者:Ophélia Le Thuc, Jacques Noël and Carole Rovère日期:08/20/2017,浏览量:159,Q&A: 0
    [Abstract] The hypothalamus is a primary brain area which, in mammals, regulates several physiological functions that are all related to maintain general homeostasis, by linking the central nervous system (CNS) and the periphery. The hypothalamus itself can be considered an endocrine brain region of some sort as it hosts in its different nuclei several kinds ...
    A High-throughput Assay for mRNA Silencing in Primary Cortical Neurons in vitro with Oligonucleotide Therapeutics
    体外使用寡核苷酸治疗药物对原代皮质神经元mRNA沉默的高通量测定
    [Abstract] Primary neurons represent an ideal cellular system for the identification of therapeutic oligonucleotides for the treatment of neurodegenerative diseases. However, due to the sensitive nature of primary cells, the transfection of small interfering RNAs (siRNA) using classical methods is laborious and often shows low efficiency. Recent progress in ...