Mario Valentino, University of Malta

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Mario Valentino

Professor, University of Malta

研究方向

Neuroscience, Neurobiology of disease

个人信息

教育背景

PhD, Max Planck Institute, 2001

实验室信息

Mario Valentino's Lab - Laboratory for the Study of Neurological Disorders

https://www.um.edu.mt/profile/mariovalentino
https://www.um.edu.mt/profile/mariovalentino

研究方向

Stroke, Two-photon imaging, White matter Injury

Research in my laboratory is directed to understanding cellular mechanisms of damage and pharmacological recovery in stroke with a special focus on hypoxic-ischemic injury of the brain's white matter. In the lab we focus on emerging concepts that drive the science of stroke in a therapeutic direction. Once considered exclusively a disorder of blood vessels, growing evidence has led to the realization that the biological processes underlying stroke are driven by the interaction of neurons, glia, vascular cells and matrix components, which actively participate in mechanisms of tissue injury and repair. There is growing recognition that approaches to reducing injury must also protect the ‘neurovascular unit’. The dynamic events can at present be followed by high resolution imaging using multiphoton microscopy that enable us to capture cellular and molecular events deep down within brain structures. Knowledge of the intricate dynamics and cross-talk between neurons, glia and the vasculature is unraveling what causes these cells to die and how they could be made to recover. One significant reason for these advances has been the development of fluorescent proteins that can be incorporated into proteins by genetic fusion to produce a fluorescent label in transgenic mice. Experimental methods used in the lab include in vivo stroke models (MCAO in mice, Photothrombotic occlusion), brain slices (OGD), optic nerve preparations, intravital imaging, electrophysiology (CAP recording), clearing techniques, immunocytochemistry and multiphoton microscopy.

发表论文

https://scholar.google.com/citations?user=Gin8s3YAAAAJ&hl=en

Mario Valentino, Maria Cristina D’Adamo Sonia Hasan,Francis Delicata,Leonardo Guasti,Claudia Duranti,Fatemah Mousalem Haidar,Annarosa Arcangeli,Paola Imbrici,Mauro Pessia. Locus Coeruleus neurons firing pattern is regulated by ERG voltage-gated K channels. International Journal of Molecular Sciences (2022); 23(23), 15334.

Christian Zammit, Mario Valentino. The Link between Cognitive Deficits and White Matter Injury in Preterms". Acta Scientific Neurology (2022): 21-22.

Cenciarini M, Valentino M, Belia S, Sforna L, Rosa P, Ronchetti S, D’Adamo M.C, Pessia M. Dexamethasone in glioblastoma Multiforme therapy: Mechanisms and controversies. Frontiers Mol. Neurosci. (2019); doi:10.3389/fnmol.2019.00065

Doyle S, Hansen DB, Vella J, Bond P, Harper G, Zammit C, Valentino M, Fern R. Vesicular glutamate release from central axons contributes to myelin damage. Nature Commun. (2018); 12;9(1):1032. doi: 10.1038/s41467-018-03427-1.

Schiavone S, Mhillaj E, Neri M, Morgese MG, Tucci P, Bove M, Valentino M, Di Giovanni G, Pomara C, Turillazzi E, Trabace L, Cuomo V. Early Loss of Blood-Brain Barrier Integrity Precedes NOX2 Elevation in the Prefrontal Cortex of an Animal Model of Psychosis. Mol. Neurobiol. (2017); 54(3):2031-2044. doi:10.1007/s12035-016-9791-8.

Casarrubea M, Davies C, Faulisi F, Pierucci M, Colangeli R, Partridge L, Chambers S, Cassar D, Valentino M, Muscat R, Benigno A, Crescimanno G, Di Giovanni G. Acute nicotine induces anxiety and disrupts temporal pattern organization of rat exploratory behavior in hole-board: a potential role for the lateral habenula. Nature. Frontiers Cell Neurosci. (2015); 1;9:197. doi: 10.3389/fncel.2015.00197. eCollection 2015.

Vella J, Zammit C, Di Giovanni G, Muscat R, Valentino M. The central role of aquaporins in the pathophysiology of ischemic stroke. Nature. Frontiers Cell Neurosci. (2015); 8;9:108. doi: 10.3389/fncel.2015.00108. eCollection 2015

Marino Gammazza A, Colangeli R, Orban G, Pierucci M, Di Gennaro G, Lo Bello M,D'Aniello A, Bucchieri F, Pomara C, Valentino M, Muscat R, Benigno A, Zummo G, deMacario EC, Cappello F, Di Giovanni G, Macario AJ. Hsp60 response in experimental and human temporal lobe epilepsy. (2015); Scientific Reports (Nature); 5:9434. doi:10.1038/srep09434.

Zammit C, Muscat R, Sani G, Pomara C, Valentino M. Cerebral white matter injury following a hypoxic/ischemic insult during the perinatal period: pathophysiology, prognostic factors, and future strategy of treatment approach. Curr Pharm Des. (2015); 21(11):1418-25. PubMed PMID: 25564391.

Laureys G, Valentino M, Demol F, Zammit C, Muscat R, Cambron M, Kooijman R, DeKeyser J. β₂-adrenergic receptors protect axons during energetic stress but do not influence basal glio-axonal lactate shuttling in mouse white matter. Neuroscience. (2014); 26;277:367-74. doi:10.1016/j.neuroscience.

Mifsud G, Zammit C, Muscat R, Di Giovanni G, Valentino M. Oligodendrocyte pathophysiology and treatment strategies in cerebral ischemia. CNS Neurosci Ther.(2014); 20(7):603-12. doi: 10.1111/cns.12263.

Orban G, Pierucci M, Benigno A, Pessia M, Galati S, Valentino M, Muscat R, Di Giovanni G. High dose of 8-OH-DPAT decreases maximal dentate gyrus activation and facilitates granular cell plasticity in vivo. Exp Brain Res. (2013); 230(4):441-51.

Zammit R, Muscat R and Valentino M. The spectrum of ischaemia-induced white matter injury varies with age. Xjenza online, (2013); (1), 1-11

Alix JJ, Zammit C, Riddle A, Meshul CK, Back SA, Valentino M, Fern R. Central axons preparing to myelinate are highly sensitive to ischemic injury. Ann Neurol. (2012); 72(6):936-51. doi: 10.1002/ana.23690.

Brunetti O, Imbrici P, Botti FM, Pettorossi VE, D'Adamo MC, Valentino M, Zammit C, Mora M, Gibertini S, Di Giovanni G, Muscat R, Pessia M. Kv1.1 knock-in ataxic mice exhibit spontaneous myokymic activity exacerbated by fatigue, ischemia and low temperature. Neurobiol Dis. (2012); 47(3):310-21.

Valentino M, Zammit C, Di Giovanni G, Muscat R. Two-photon microscopy- sequential imaging studies in vivo. MMJ (2011); 23, (3), 38-44. Special Issue, Neuroscience.

Valentino M. Shedding Light on Brain Injury. Research Matters (2011); 10-13. ISBN: 978-99957-0-162-8.

Pierucci M, Galati S, Valentino M, Di Matteo V, Benigno A, Pitruzzella A, Muscat R, Di Giovanni G. Nitric oxide modulation of the basal ganglia circuitry: therapeutic implication for Parkinson's disease and other motor disorders. CNS & Neurological Disorders. Drug Targets, (2011);10(7):777-791.

Zammit C, Valentino M, Muscat R. Vulnerability of white matter to ischemia varies during development. MMJ (2011); 23,(3),45-51.

Pierucci M, Pitruzella A, Valentino M, Zammit C, Muscat R, Beningno A, Di Giovanni G. (2011). Lateral habenula contribution in nicotine addiction. Focus on dopamine, GABA and serotonin interactions. MMJ (2011); 23 (3) 52-57.

Ness JK, Valentino M, McIver SR, Goldberg MP. Identification of oligodendrocytes in experimental disease models. Glia. (2005); 50(4):321-8.

Toyota S, Graf.R, Valentino M, Fujinaka T, Yoshimine T, Heiss W-D. Neurochemical monitoring in malignant edema. Japanese Journal of Stroke. (2004); 26(4), 524-529.

Heiss WD, Dohmen C, Sobesky J, Kracht L, Bosche B, Staub F, Toyota S, Valentino M, Graf R. Identification of malignant brain edema after hemispheric stroke by PET-imaging and microdialysis. Acta Neurochir Suppl. (2003); 86:237-40. DOI:10.1007/978-3-7091-0651-8_51.

Toyota S, Graf R, Valentino M, Yoshimine T, Heiss WD. Prediction of malignant infarction: perifocal neurochemical monitoring following prolonged MCA occlusion in cats. Acta Neurochir. Suppl. (2003); 86:153-7.

Toyota S, Graf R, Valentino M, Yoshimine T, Heiss WD. Malignant infarction in cats after prolonged middle cerebral artery occlusion: glutamate elevation related to decrease of cerebral perfusion pressure. Stroke. (2002); 33(5):1383-91.

Toyota S, Graf R, Dohmen C, Valentino M, Grond M, Wienhard K, Heiss WD.
Elevation of extracellular glutamate in the final, ischemic stage of progressive epidural
mass lesion in cats. J Neurotrauma. (2001); 18(12):1349-57.

Toyota S, Valentino M, Graf R, Heiss W-D. Malignant edema formation following transient MCA occlusion: decrease of cerebral perfusion pressure causes secondary elevation of glutamate. J. Cereb. Blood Flow Metab. (2001); Suppl 1.

Valentino M, Graf R, Toyota S, Heiss W-D. Glutamate and purine catabolites in relation to free radical production during focal ischemia-reperfusion: an in vivo study in cats. J. Cereb. Blood Flow Metab. (2001); 21, 287-92.

Graf R, Toyota S, Valentino M, Dohmen C, Heiss W-D. Malignant infarction in cats following prolonged middle cerebral artery occlusion: volumes of severe blood flow reduction predict fatal outcome. J. Cereb. Blood Flow Metab. (2001); Suppl 1.

Graf R, Toyota S, Dohmen C, Valentino M,Wienhard K , Heiss W-D. Extracellular glutamate accumulates only in final, ischemic stage of progressive epidural mass lesion in cats. J. Cereb. Blood Flow Metab. (2001); Suppl 1.

Valentino M and Graf R. Emerging insights into the genesis of cerebral ischaemia. Xjenza, (1999); Vol4, 4-11.

Borg I, Valentino M, Fiorini A, Felice AE. Hb Setif [alpha 94(G1)Asp-->Tyr]in Malta. Hemoglobin. (1997); 21(1):91-6.

The front cover figure of CNS Neuroscience & Therapeutics, Special issue: Interdisciplinary Approaches for Neuropathology, Vol. 20,(7), 2014 was dedicated to our article: Mifsud et al., 2014.

The front cover image on Xjenza Online Vol3, (1), 2015) was chosen through an internal jury.

Acknowledgement by Anton Sammut for my review contribution to the book: Consciousness. The Concept of Mind, 2016. ISBN 9789995710330.

BOOK CHAPTERS

E. Esposito, V. Di Matteo, M. Pierucci, A. Benigno, M. Pessia, A. Pitruzzella, C. Zammit, M. Valentino, R. Muscat, G. Di Giovanni. Serotonin-Dopamine interaction in Nicotine Addiction: Focus on 5-HT2C Receptors (2013); In:Nicotine Addiction: Prevention, Health Effects and Treatment Options, Nova Publisher, New York, ISBN:978-1-62081, 25-52.

Heiss W-D, Dohmen C, Sobesky J, Kracht L, Bosche B, Staub F, Toyota S, Valentino M, Graf R. Prediction of malignant infarction: perifocal neurochemical monitoring following prolonged MCA occlusion in cats (2003); In: Brain Edema XII R. Springer-Verlag, 237-240, ISBN-10: 3211009191.

Graf R, Nozaki H, Toyota S, Ohta K, Valentino M, Kumura E, Dohmen C, Sakaki T, Rosner G, Heiss W-D. Ischemic depression of neuronal activity: real time comparison between DC potential changes and alterations of ion and transmitter homeostasis. In:Tomita M, Kanno I, Hamel E, eds. Brain Activation and CBF Control, (2002); Amsterdam: Elsevier, 465-474. ISBN:0444508740.