[反馈 1] The protocol was very helpful. I ended up using a few modifications for the conditions in my lab. Specifically, I dissolved the X-Gluc in Methanol, not in DMF. I prepared a 20mM stock and I used a final concentration of 2mM instead of 1mM, this was likely necessary because the reagents in my lab might have been too old. I also reduced the potassium ferricyanide and potassium ferrocyanide to 1mM, and the Triton X-100 to 0.05%. I used 50mM Sodium citrate buffer pH 7.2 instead of phosphate buffer. Also, depending on the promoter that I was using, I had to incubate at 37C in the dark for up to 48h. But, at the end of the day, the results were beautiful.