Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:

Socodato, R, Summavielle, T and Relvas, J(2020). Brain tissue preparation and immunohistochemistry. Bio-protocol Preprint. bio-protocol.org/prep567.
Socodato, R., Henriques, J. F., Portugal, C. C., Almeida, T. O., Tedim-Moreira, J., Alves, R. L., Canedo, T., Silva, C., Magalhães, A., Summavielle, T. and Relvas, J. B.(2020). Daily alcohol intake triggers aberrant synaptic pruning leading to synapse loss and anxiety-like behavior . Science Signaling 13(650). DOI: 10.1126/scisignal.aba5754