TM
Taro Mannen
  • Institute for Genetic Medicine, Hokkaido University, Japan
研究方向
  • Cell biology
RNase Sensitivity Screening for Nuclear Bodies with RNA Scaffolds in Mammalian Cells
哺乳动物细胞中含RNA支架的核体的核糖核酸酶敏感性筛选
作者:Taro Mannen and Tetsuro Hirose日期:04/20/2017,浏览量:9498,Q&A: 0
The mammalian cell nucleus is highly organized and contains membraneless nuclear bodies (NBs) characterized by distinct resident factors. The NBs are thought to serve as sites for biogenesis and storage of certain RNA and protein factors as well as assembly of ribonucleoprotein complexes. Some NBs are formed with architectural RNAs (arcRNAs) as their structural scaffolds and additional NBs likely remain unidentified in mammalian cells. Here, we describe an experimental protocol to search for new NBs built on certain arcRNAs. RNase-sensitive NBs were identified by monitoring nuclear foci visualized by tagging thousands of human cDNA products.