Ionut Dumitru
  • Department of Clinical Neurobiology at the German Cancer Research Center (DKFZ) and the Medical Faculty of the Heidelberg University, Germany
研究方向
  • Neuroscience
Mouse Subependymal Zone Explants Cultured on Primary Astrocytes
小鼠室管膜下区外植体在原代星形胶质细胞中的培养
作者:Ionut Dumitru, Hannah Monyer and Julieta Alfonso日期:07/20/2016,浏览量:7739,Q&A: 0
Neuroblast migration is a major component in the process of neuronal circuit assembly. In the rodent postnatal brain, the subependymal zone (SEZ) is the largest neurogenic niche where new neurons are born throughout life. These cells migrate several millimeters following a specific pathway called the rostral migratory stream (RMS) towards the olfactory bulb. Once they reach their final destination, they differentiate mainly as interneurons and integrate into already established neuronal circuits. Along the RMS, neuroblasts use a particular mode of migration known as chain migration. They stay attached to each other while migrating and are intimately associated with adjacent astrocytic processes. To dissect the molecular and cellular signals involved in neuroblast migration, we developed an in vitro system consisting of SEZ-derived explants co-cultured on top of an astrocyte monolayer.