CJ
Congshan Jiang
  • Faculty, Xi'an Children's Hospital, Affiliated Children's Hospital of Xi'an Jiaotong University Xi'an
研究方向
  • Immunology, Molecular Biology, Neuroscience, Stem Cell
Successful Transfection of MicroRNA Mimics or Inhibitors in a Regular Cell Line and in Primary Cells Derived from Patients with Rheumatoid Arthritis
在常规细胞系和源自类风湿性关节炎患者的原代细胞中成功转染 MicroRNA 模拟物或抑制剂
作者:Si Wang, Jing Xu, Yuanxu Guo, Yongsong Cai, Wenhua Zhu, Liesu Meng, Congshan Jiang and Shemin Lu日期:09/20/2023,浏览量:565,Q&A: 0

The transfection of microRNA (miRNA) mimics and inhibitors can lead to the gain and loss of intracellular miRNA function, helping us better understand the role of miRNA during gene expression regulation under specific physical conditions. Our previous research has confirmed the efficiency and convenience of using liposomes to transfect miRNA mimics or inhibitors. This work uses miR-424 as an example, to provide a detailed introduction for the transfection process of miRNA mimics and inhibitors in the regular SW982 cell line and primary rheumatoid arthritis synovial fibroblasts (RASF) cells from patients by using lipofection, which can also serve as a reference to miRNA transfection in other cell lines.


Key features

• MiRNA mimics and inhibitors transfection in regular SW982 cell line and primary RASF cells.

• Treatment and culture of RASF primary cells before transfection.

Using liposomes for transfection purposes.