Rice blast and wheat blast caused by Magnaporthe oryzae is a serious threat to rice and wheat production. Appropriate methods for observing M. oryzae infection process are important for study of the fungal infection mechanisms, plant resistance reactions, and host-M. oryzae interactions. The rice leaf sheath is commonly used to inoculate M. oryzae for observing the infection process, however, this method is a time-consuming and high technical work. Here, we describe an easier solution to observe M. oryzae infection process on barley leaves.