MN
Monford Paul Abishek N
  • Post-Doc, Chungnam National University Yuseong, Daejeon
研究方向
  • Cell Biology, Microbiology, Molecular Biology
An in vitro Assay of mRNA 3’ end Using the E. coli Cell-free Expression System
使用大肠杆菌无细胞表达系统对 mRNA 3' 端进行体外测定
作者:Monford Paul Abishek N and Heon M. Lim日期:02/20/2022,浏览量:2152,Q&A: 0

At the end of about 80% of the operon in Escherichia coli, translation termination decouples transcription, leading to Rho-dependent transcription termination (RDT). However, no in vitro or in vivo assay system has proven to be good enough to see

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Visualization of RNA 3’ ends in Escherichia coli Using 3’ RACE Combined with Primer Extension
3'RACE联合引物延伸法检测大肠埃希氏菌RNA 3'末端
作者:Xun Wang, Heung Jin Jeon, Monford Paul Abishek N, Jin He and Heon M. Lim日期:03/05/2018,浏览量:7864,Q&A: 0
In this assay, 3’ RACE (Rapid Amplification of cDNA 3’ Ends) followed by PE (primer extension), abbreviated as 3’ RACE-PE is used to identify the mRNA 3’ ends. The following protocol describes the amplification of the mRNA 3’ ends at the galactose ...