Yang Liu, Medicine, University of Pittsburgh

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Yang Liu

Associate professor, Medicine, University of Pittsburgh

研究方向

Immunofluorescent Staining of Mouse Intestinal Stem Cells
小鼠小肠干细胞的免疫荧光染色

作者：Kevin P. O’Rourke, Lukas E Dow and Scott W Lowe，日期：02/20/2016，浏览量：32965，Q&A： 1

Immunofluorescent staining of organoids can be performed to visualize molecular markers of cell behavior. For example, cell proliferation marked by incorporation of nucleotide (EdU), or to observe markers of intestinal differentiation including ...

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Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells
小鼠小肠干细胞的分离、培养和维持

作者：Kevin P. O’Rourke, Sarah Ackerman, Lukas E Dow and Scott W Lowe，日期：02/20/2016，浏览量：28372，Q&A： 6

In this protocol we describe our modifications to a method to isolate, culture and maintain mouse intestinal stem cells as crypt-villus forming organoids. These cells, isolated either from the small or large intestine, maintain self-renewal and ...

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