教育背景

Ph.D., Federal University of Rio de Janeiro (UFRJ), 2023

实验室信息

I am a Postdoctoral Fellow in the Encalada lab (https://www.encalada.scripps.edu/) at the Scripps Research Institute.

研究方向

RESEARCH INTERESTS
✓ Molecular mechanisms underlying proteinopathies, and liquid-to-solid transition of aggregation-prone
proteins related to neurodegenerative diseases.
✓ Biochemical, structural, and in cellulo characterization of biomolecular condensates and aggregates drove by intrinsically disordered proteins and its modulators (metal ions, DNA/RNA, protein clients,
physicochemical factors).

EXPERTISE
✓ Recombinant protein production in E. coli and heterologous expression in mammalian cells.
✓ Molecular cloning techniques.
✓ Protein purification (e.g. chromatographic methods such as affinity, ion exchange, desalting and size
exclusion, ammonium sulphate precipitation, dialysis, ultrafiltration).
✓ Biochemical and Biophysical characterization (denaturing and native gel electrophoresis, agarose gels, Western blots, Circular Dichroism, Fluorescence, Spectrophotometry, Dynamic Light Scattering).
✓ Microscopy (DIC, Fluorescence, Confocal, TIRF, FRAP, TEM).
✓ Protein-ligand interactions (SPR, ITC, SEC-HPLC, Native PAGE).
✓ Cell-based assays involving protein expression in cell lines and neonate primary mouse neurons.

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