分子生物学

分类

    Protocol for RNA-seq Expression Analysis in Yeast
    酵母RNA-seq表达分析方法
    作者:  Stefan Bohn, 日期: 09/20/2021, 浏览量: 541, Q&A: 0

    Genome-wide sequencing of RNA (RNA-seq) has become an inexpensive tool to gain key insights into cellular and disease mechanisms. Sample preparation and sequencing are streamlined and allow the acquisition of hundreds of gene expression profiles in

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    Construction of DNA/RNA Triplex Helices Based on GAA/TTC Trinucleotide Repeats
    基于GAA/TTC三核苷酸重复序列构建DNA/RNA三链螺旋
    作者:  Jiahui Zhang, Ashkan Fakharzadeh, Feng Pan, Christopher RolandCeleste Sagui, 日期: 09/20/2021, 浏览量: 464, Q&A: 0

    Atypical DNA and RNA secondary structures play a crucial role in simple sequence repeat (SSR) diseases, which are associated with a class of neurological and neuromuscular disorders known as “anticipation diseases,” where the age of

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    Quantitative Analysis of RNA Editing at Specific Sites in Plant Mitochondria or Chloroplasts Using DNA Sequencing
    利用DNA测序对植物线粒体或叶绿体特定位点RNA编辑的定量分析
    作者:  Yang YangWeixing Shan, 日期: 09/20/2021, 浏览量: 392, Q&A: 0

    Cytidine-to-uridine (C-to-U) RNA editing is one of the most important post-transcriptional RNA processing in plant mitochondria and chloroplasts. Several techniques have been developed to detect the RNA editing efficiency in plant mitochondria and

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    Direct-TRI: High-throughput RNA-extracting Method for all Stages of Zebrafish Development
    Direct-TRI: 斑马鱼发育各阶段高通量RNA提取方法
    作者:  Kota Ujibe, Kanako Nishimura, Makoto KashimaHiromi Hirata, 日期: 09/05/2021, 浏览量: 634, Q&A: 0

    Recent popularization of next-generation sequencing enables conducting easy transcriptome analysis. Nevertheless, substantial RNA isolation work prior to RNA sequencing, as well as the high cost involved, still makes the routine use of large-scale

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    Preparation and Characterization of Internally Modified DNA Templates for Chemical Transcription Roadblocking
    用于化学转录障碍的内部修饰 DNA 模板的制备和特征分析
    作者:  Eric J. Strobel, 日期: 09/05/2021, 浏览量: 488, Q&A: 0

    Site-specific transcription arrest is the basis of emerging technologies that assess nascent RNA structure and function. Cotranscriptionally folded RNA can be displayed from an arrested RNA polymerase (RNAP) for biochemical manipulations by halting

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    U2.3 Precursor Small Nuclear RNA in vitro Processing Assay
    U2.3前体小核 RNA体外加工试验
    作者:  Chan Lin, Yujie Feng, Xueyan Peng, Jiaming Wu, Weili WangYunfeng Liu, 日期: 09/05/2021, 浏览量: 399, Q&A: 0

    Small nuclear RNAs (snRNAs) are vital for eukaryotic cell activities and play important roles in pre-mRNA splicing. The molecular mechanism underlying the transcription of snRNA, regulated via upstream/downstream cis-elements and relevant

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    In situ Hybridization of miRNAs in Human Embryonic Kidney and Human Pluripotent Stem Cell-derived Kidney Organoids
    在人胚肾和人多能干细胞衍生的肾类器官中微小RNA的原位杂交
    作者:  Filipa M. Lopes, Susan J. KimberIoannis Bantounas, 日期: 09/05/2021, 浏览量: 557, Q&A: 0

    MicroRNAs are small RNAs that negatively regulate gene expression and play an important role in fine-tuning molecular pathways during development. There is increasing interest in studying their function in the kidney, but the majority of studies to

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    小鼠脑切片原位杂交
    In-situ Hybridization for Mouse Brain Sections
    作者:  应玥, 蔡予琦, 吴锦云何苗, 日期: 08/10/2021, 浏览量: 263, Q&A: 0
    摘要:原位杂交 (In-situ hybridization, ISH) ...
    Isolation of Nuclei from Mouse Dorsal Root Ganglia for Single-nucleus Genomics
    小鼠背根神经节细胞核的分离及其单核基因组学研究
    作者:  Lite Yang, Ivan Tochitsky, Clifford J. WoolfWilliam Renthal, 日期: 08/05/2021, 浏览量: 807, Q&A: 0

    Primary somatosensory neurons, whose cell bodies reside in the dorsal root ganglion (DRG) and trigeminal ganglion, are specialized to transmit sensory information from the periphery to the central nervous system. Our molecular understanding of

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    Efficient and Rapid Analysis of Polysomes and Ribosomal Subunits in Cells and Tissues Using Ribo Mega-SEC
    利用Ribo Mega-SEC高效快速分析细胞和组织中的多聚体和核糖体亚基

    Polysome profile analysis is a popular method for separating polysomes and ribosomal subunits and is typically achieved using a sucrose density gradient (SDG). This has remained the gold standard method since ribosomes were first discovered;

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