植物科学

分类

    Safe DNA-extraction Protocol Suitable for Studying Tree-fungus Interactions
    适于研究树-真菌互作的安全DNA提取方法
    作者:  Susanna Keriö, Eeva TerhonenJared M. LeBoldus, 日期: 06/05/2020, 浏览量: 4341, Q&A: 0
    We present a safe and low-cost method suitable for DNA extraction from mycelium and tree tissue samples. After sample preparation, the extraction takes about 60 min. Method performance was tested by extracting DNA from various tree tissue samples ...
    CTAB法微量提取柑橘基因组DNA
    Citrus Genomic DNA Microextraction Using CTAB Method
    作者:  徐远涛, 余惠文, 程运江, 徐强邓秀新, 日期: 07/10/2018, 浏览量: 3646, Q&A: 0
    实验原理:利用CTAB (十六烷基三甲基溴化铵) 作为一种阳离子去污剂,在高离子强度的溶液中与蛋白质和多聚糖形成复合物,而在低离子强度溶液中可以沉淀核酸与酸性多聚糖的特性将DNA从柑橘组织样品中分离出来。
    实验目的:快速 ...
    高纯度柑橘基因组DNA提取与检测
    Extraction and Detection of High-purity Genomic DNA from Citrus
    作者:  徐远涛, 余惠文, 程运江, 徐强邓秀新, 日期: 07/10/2018, 浏览量: 2237, Q&A: 0
    实验原理:利用CTAB (十六烷基三甲基溴化铵) 作为一种阳离子去污剂,在高离子强度的溶液中与蛋白质和多聚糖形成复合物,而在低离子强度溶液中可以沉淀核酸与酸性多聚糖的特性将DNA从柑橘组织样品中分离出来 ...
    An Optimized CTAB Method for Genomic DNA Extraction from Freshly-picked Pinnae of Fern, Adiantum capillus-veneris L.
    一种从新鲜收取的铁线蕨样品中提取基因组DNA 的优化CTAB法
    作者:  Yi Shu, Jin Wan-Ting, Yuan Ya-NingFang Yu-Han, 日期: 07/05/2018, 浏览量: 14508, Q&A: 0
    As the sister clade of seed plants, ferns are significant materials for plant phylogeny research. However, the genomic DNA extraction protocol for fern samples like modified CTAB method still lacks robustness. Here, we found that the amount and ...
    Isolation of Genomic DNA from Chlamydomonas reinhardtii
    莱茵衣藻基因组DNA的分离
    作者:  Tyler M Wittkopp, 日期: 05/05/2018, 浏览量: 11305, Q&A: 0
    Chlamydomonas reinhardtii is a soil-dwelling eukaryotic green alga that is widely used as a laboratory model organism for research on photosynthesis, ciliary biology, lipid metabolism and many other aspects of cell biology and physiology. ...
    Small-scale DNA Extraction Method for Maize and Other Plants
    玉米及其他植物DNA小提法
    作者:  China Lunde, 日期: 03/20/2018, 浏览量: 6234, Q&A: 1
    Corn, or maize, is a major cereal crop and model monocot. Two of its benefits are ease of pollination and tractable genetics. For a comparison of development between maize and the dicot model, Arabidopsis, see Lunde and Hake, 2005. For an ...
    Rolling Circle Amplification to Screen Yam Germplasm for Badnavirus Infections and to Amplify and Characterise Novel Badnavirus Genomes
    滚换扩增筛选山药Badnavirus感染种质并扩增鉴定新型Badnavirus基因组
    作者:  Moritz Bömer, Aliyu A. Turaki, Ajith I. Rathnayake, Gonçalo Silva, P. Lava KumarSusan E. Seal, 日期: 01/05/2018, 浏览量: 7705, Q&A: 0
    Since the first discovery of badnaviruses (family Caulimoviridae, genus Badnavirus) in yam (Dioscorea spp.) germplasm in the 1970s (Harrison and Roberts, 1973), several hundred partial badnavirus reverse transcriptase ...
    Determining Genome Size from Spores of Seedless Vascular Plants
    无种子维管束植物孢子基因组大小的测定
    作者:  Li-Yaung KuoYao-Moan Huang , 日期: 06/05/2017, 浏览量: 7089, Q&A: 0
    Seedless vascular plants, including ferns and lycophytes, produce spores to initiate the gametophyte stage and to complete sexual reproduction. Approximately 10% of them are apomictic through the production of genomic unreduced spores. Being able to ...
    Experimental Pipeline for SNP and SSR Discovery and Genotyping Analysis of Mango (Mangifera indica L.)
    芒果SNP和SSR的发现和基因分型分析的实验方法
    Establishing a reservoir of polymorphic markers is an important key for marker-assisted breeding. Many crops are still lack of such genomic infrastructure. Single nucleotide polymorphisms (SNPs) and simple sequence repeats (SSRs) are useful as ...
    Genomic DNA Extraction and Genotyping of Dictyochloropsis Green Algae Strains
    Dictyochloropsis绿藻菌株的基因组DNA提取和基因分型
    作者:  Francesco Dal Grande, Carolina Cornejo, Christoph ScheideggerAndreas Beck, 日期: 08/05/2015, 浏览量: 11026, Q&A: 0
    Dictyochloropsis is an ecologically important genus of free-living and symbiotic green algae. Representatives of this genus are horizontally transmitted among several fungi of the family Lobariaceae, thus forming photobiont-mediated guilds. ...