生物物理学

分类

    Using Atomic Force Microscopy to Study the Real Time Dynamics of DNA Unwinding by Mitochondrial Twinkle Helicase
    使用原子力显微镜研究线粒体闪烁解旋酶对DNA解旋的实时动力学
    作者:  Parminder Kaur, Hai Pan, Matthew J. Longley, William C. CopelandHong Wang, 日期: 09/05/2021, 浏览量: 698, Q&A: 0

    Understanding the structure and dynamics of DNA-protein interactions during DNA replication is crucial for elucidating the origins of disorders arising from its dysfunction. In this study, we employed Atomic Force Microscopy as a single-molecule

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    Unraveling the Physicochemical Determinants of Protein Liquid-liquid Phase Separation by Nanoscale Infrared Vibrational Spectroscopy
    利用纳米尺度红外振动光谱揭示蛋白质液-液相分离的理化决定因素
    作者:  Francesco S. Ruggeri, Alyssa M. Miller, Michele VendruscoloTuomas P. J. Knowles, 日期: 08/20/2021, 浏览量: 847, Q&A: 0

    The phenomenon of reversible liquid-liquid phase separation of proteins underlies the formation of membraneless organelles, which are crucial for cellular processes such as signalling and transport. In addition, it is also of great interest to

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    Synchronized Real-time Measurement of Sec-mediated Protein Translocation
    Sec介导的蛋白质易位的同步实时测量
    作者:  Riti Gupta, Dmitri ToptyginChristian M. Kaiser, 日期: 08/20/2021, 浏览量: 615, Q&A: 0

    The Sec translocon, consisting of a heterotrimeric transmembrane channel (SecYEG) and an associated ATPase (SecA), catalyzes the export of unfolded proteins from the cytosol in bacteria. Kinetically resolving protein translocation at high resolution

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    Cell-attached and Whole-cell Patch-clamp Recordings of Dopamine Neurons in the Substantia Nigra Pars Compacta of Mouse Brain Slices
    小鼠脑片黑质致密部多巴胺神经元的细胞贴附和全细胞膜片钳记录
    作者:  Stefano Cattaneo, Maria Regoni, Jenny SassoneStefano Taverna, 日期: 08/05/2021, 浏览量: 746, Q&A: 0

    The Substantia Nigra pars compacta (SNc) is a midbrain dopaminergic nucleus that plays a key role in modulating motor and cognitive functions. It is crucially involved in several disorders, particularly Parkinson’s disease, which is

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    Purification of Mitochondrial Ribosomes with the Translocase Oxa1L from HEK Cells
    用转位酶Oxa1L纯化HEK细胞线粒体核糖体
    作者:  Hanting YangNirupa Desai, 日期: 08/05/2021, 浏览量: 955, Q&A: 0

    Mitochondrial ribosomes (mitoribosomes) perform protein synthesis inside mitochondria, the organelles responsible for energy conversion and adenosine triphosphate (ATP) production in eukaryotic cells. To investigate their functions and structures,

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    Purification and Cryo-electron Microscopy Analysis of Plant Mitochondrial Ribosomes
    植物线粒体核糖体的纯化和低温电镜分析
    作者:  Florent Waltz, Philippe GiegéYaser Hashem, 日期: 08/05/2021, 浏览量: 757, Q&A: 0

    Plants make up by far the largest part of biomass on Earth. They are the primary source of food and the basis of most drugs used for medicinal purposes. Similarly to all eukaryotes, plant cells also use mitochondria for energy production. Among

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    Ion Transport Activity Assay for Microbial Rhodopsin Expressed in Escherichia coli Cells
    大肠杆菌表达的微生物紫红质离子转运活性测定
    作者:  Masae Konno, Keiichi InoueHideki Kandori, 日期: 08/05/2021, 浏览量: 635, Q&A: 0

    Microbial rhodopsins have diverse functions, including roles as light-driven ion pumps, light-gated ion channels, photosensors, and light-regulated enzymes. As the number of rhodopsin-like genes identified has increased in recent years, so has the

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    Spatiotemporal Quantification of Cytosolic pH in Arabidopsis Pollen Tubes
    拟南芥花粉管胞质pH的时空定量研究
    作者:  Maria Teresa Portes, Daniel S.C. DamineliJosé A. Feijó, 日期: 07/20/2021, 浏览量: 796, Q&A: 0

    Ion-specific probes and fluorescent indicators have been key in establishing the role of ion signaling, namely calcium, protons, and anions, in plant development, providing a robust approach for monitoring spatiotemporal changes in intracellular ion

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    Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts
    膜片钳移液管重复使用的快速酶清洗方法:通过消除膜片钳尝试之间的手动移液管更换来提高吞吐量
    作者:  Corey R. Landry, Mighten C. Yip, Ilya Kolb, William A. Stoy, Mercedes M. GonzalezCraig R. Forest, 日期: 07/20/2021, 浏览量: 851, Q&A: 0

    The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and

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    A Multi-color Bicistronic Biosensor to Compare the Translation Dynamics of Different Open Reading Frames at Single-molecule Resolution in Live Cells
    一种多色双顺反子生物传感器比较活细胞中不同开放阅读框在单分子分辨率下的翻译动力学
    作者:  Amanda L. Koch, Tatsuya MorisakiTimothy J. Stasevich, 日期: 07/20/2021, 浏览量: 1119, Q&A: 0

    Here, we describe how to image and quantitate the translation dynamics of a bicistronic biosensor that we recently created to fairly compare cap-dependent and IRES-mediated translation at single-molecule resolution in live human cells. This

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