细胞生物学

分类

    Microscopic Detection of ASC Inflammasomes in Bone Marrow Derived Macrophages Post Stimulation
    刺激后骨髓源性巨噬细胞中ASC炎症小体的显微检测
    作者:  Longjun Li, Rudi MaoXiaopeng Qi, 日期: 09/05/2021, 浏览量: 613, Q&A: 0

    An inflammasome is an intracellular multiprotein complex that plays important roles in host defense and inflammatory responses. Inflammasomes are typically composed of the adaptor protein apoptosis-associated speck-like protein containing a CARD

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    Isolation and Quantification of Mouse γδT-cells in vitro and in vivo
    体外和体内小鼠γδT细胞的分离和定量
    作者:  Isha Rana, Krithika Badarinath, Ravindra K. ZirmireColin Jamora, 日期: 09/05/2021, 浏览量: 716, Q&A: 0

    The skin plays an important role in protecting the body from pathogens and chemicals in the external environment. Upon injury, a healing program is rapidly initiated and involves extensive intercellular communication to restore tissue homeostasis.

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    Plasmodium cynomolgi Berok Growth Inhibition Assay by Thiol-reactive Probe Based Flow Cytometric Measurement
    硫醇反应探针流式细胞术检测食蟹猴疟原虫生长抑制
    作者:  Jessica Jie Ying Ong, Bruce RussellJin-Hee Han, 日期: 09/05/2021, 浏览量: 601, Q&A: 0

    The relapsing malaria species, Plasmodium vivax, is the most widely distributed and difficult-to-treat cause of human malaria. The merozoites of P. vivax preferentially invade ephemeral human CD71+ reticulocytes (nascent reticulocytes), thereby

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    Cytoduction and Plasmiduction in Yeast
    酵母细胞诱导中的细胞诱导和质粒诱导
    作者:  Jane E. DorweilerAnita L. Manogaran, 日期: 09/05/2021, 浏览量: 675, Q&A: 0

    Cytoduction, and a related technique referred to as plasmiduction, have facilitated substantial advancements in the field of yeast prion biology by providing a streamlined method of transferring prions from one yeast strain to another. Prions are

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    Isolation and Characterization of Membrane Vesicles from Lactobacillus Species
    乳酸杆菌属膜囊泡中的分离和特性研究
    作者:  Julie C. Caruana, Scott N. DeanScott A. Walper, 日期: 09/05/2021, 浏览量: 719, Q&A: 0

    Throughout their life cycle, bacteria shed portions of their outermost membrane comprised of proteins, lipids, and a diversity of other biomolecules. These biological nanoparticles have been shown to have a range of highly diverse biological

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    One-step White Blood Cell Extracellular Staining Method for Flow Cytometry
    流式细胞术白细胞细胞外一步染色法
    作者:  Ines Ait Belkacem, Pénélope Bourgoin, Jean Marc Busnel, Franck GallandFabrice Malergue, 日期: 08/20/2021, 浏览量: 608, Q&A: 0

    Flow cytometry is a powerful analytical technique that is increasingly used in scientific investigations and healthcare; however, it requires time-consuming, multi-step sample procedures, which limits its use to specialized laboratories. In this

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    Preparation of Zygotes and Embryos of the Kelp Saccharina latissima for Cell Biology Approaches
    用细胞生物学方法制备糖精海带受精卵和胚胎
    作者:  Ioannis Theodorou, Hilde-Gunn Opsahl-SortebergBénédicte Charrier, 日期: 08/20/2021, 浏览量: 164, Q&A: 0

    Embryogenesis and early development in kelps are poorly studied and understood. Cultivation protocols focus mostly either on the preparation of large volumes of vegetative gametophytes or on the production of conspicuous juvenile sporophytes as

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    A Novel Method to Make Polyacrylamide Gels with Mechanical Properties Resembling those of Biological Tissues
    一种制备具有类似生物组织力学性能的聚丙烯酰胺凝胶的新方法  
    作者:  Katarzyna Pogoda, Elisabeth E. CharrierPaul A. Janmey, 日期: 08/20/2021, 浏览量: 892, Q&A: 0

    Studies characterizing how cells respond to the mechanical properties of their environment have been enabled by the use of soft elastomers and hydrogels as substrates for cell culture. A limitation of most such substrates is that, although

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    A Novel Method to Map Small RNAs with High Resolution
    一种新的高分辨率小RNA图谱绘制方法
    作者:  Kun Huang, Feray Demirci, Blake C. MeyersJeffrey L. Caplan, 日期: 08/20/2021, 浏览量: 545, Q&A: 0

    Analyzing cellular structures and the relative location of molecules is essential for addressing biological questions. Super-resolution microscopy techniques that bypass the light diffraction limit have become increasingly popular to study cellular

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    Preparation of Drosophila Larval Blood Cells for Single-cell RNA Sequencing
    果蝇幼虫血细胞单细胞RNA测序的制备
    作者:  Sudhir Gopal TattikotaNorbert Perrimon, 日期: 08/20/2021, 浏览量: 465, Q&A: 0

    Recent advances in single-cell RNA-sequencing (scRNA-seq) technologies provide unprecedented opportunities to identify new cell types and characterize cell states. One of the most important requirements for performing scRNA-seq is to obtain

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