发育生物学

分类

    A Methodology for the Enzymatic Isolation of Embryonic Hypothalamus Tissue and Its Acute or Post-Culture Analysis by Multiplex Hybridisation Chain Reaction
    胚胎下丘脑组织的酶分离方法及通过多重杂交链式反应进行的急性或培养后分析
    作者:  Kavitha ChinnaiyaMarysia Placzek, 日期: 12/05/2023, 浏览量: 63, Q&A: 0

    The hypothalamus is an evolutionarily ancient part of the vertebrate ventral forebrain that integrates the dialogue between environment, peripheral body, and brain to centrally govern an array of physiologies and behaviours. Characterizing the

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    An Improved Protocol for the Matrigel Duplex Assay: A Method to Measure Retinal Angiogenesis
    Matrigel Duplex分析的改进方案: 测量视网膜血管生成的方法

    Neovascular diseases of the retina, such as diabetic retinopathy (DR) and age-related macular degeneration (AMD), are proliferative retinopathies involving the growth of new blood vessels on the retina, which in turn causes impairment and potential

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    Preparation of Cardiac Extracts from Embryonal Hearts to Capture RNA–protein Interactions by CLIP
    制备胚胎心脏提取物以通过 CLIP 捕获 RNA-蛋白质相互作用
    作者:  Giulia Buonaiuto, Valeria Taliani, Carmine Nicoletti, Fabio DesideriMonica Ballarino, 日期: 10/20/2023, 浏览量: 311, Q&A: 0

    The interaction of RNA with specific RNA-binding proteins (RBP) leads to the establishment of complex regulatory networks through which gene expression is controlled. Careful consideration should be given to the exact environment where a given

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    A Rapid and Simple Procedure for the Isolation of Embryonic Cells from Fish Eggs
    一种快速简便的从鱼卵中分离胚胎细胞的方法
    作者:  Vasily Golotin, Anatoly Lyutikov, Tatiana Filatova, Vladimir SharoykoOlga Apalikova, 日期: 10/05/2023, 浏览量: 201, Q&A: 0

    Fertilized teleost fish eggs are a complex formation, in which dividing cells arelocated in a small point in the entire volume of eggs. Isolating embryonic cellscan be considered a necessary step in the research of developmentalpeculiarities of fish

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    Isolation of Embryonic Cardiomyocytes and Cell Proliferation Assay Using Genetically Engineered Reporter Mouse Model
    使用基因工程报告小鼠模型分离胚胎心肌细胞并进行细胞增殖测定
    作者:  Maren Beall, Deqiang LiJihyun Jang, 日期: 09/05/2023, 浏览量: 467, Q&A: 0

    Congenital heart disease (CHD) is often associated with myogenic defects. During heart development, cardiomyocyte growth requires essential cues from extrinsic factors such as insulin-like growth factor 2 (IGF-2). To determine whether and how growth

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    Spatial Centrosome Proteomic Profiling of Human iPSC-derived Neural Cells
    人 iPSC 衍生神经细胞的空间中心体蛋白质组学分析
    作者:  Fatma UzbasAdam C. O’Neill, 日期: 09/05/2023, 浏览量: 444, Q&A: 0

    The centrosome governs many pan-cellular processes including cell division, migration, and cilium formation. However, very little is known about its cell type-specific protein composition and the sub-organellar domains where these protein

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    Stereotactic Delivery of Helper-dependent Adenoviral Viral Vectors at Distinct Developmental Time Points to Perform Age-dependent Molecular Manipulations of the Mouse Calyx of Held
    在不同发育时间点立体定向递送依赖性腺病毒载体,对小鼠花萼突触进行年龄依赖性分子操作
    作者:  Christian Keine, Mohammed Al-Yaari, Tamara RadulovicSamuel M. Young Jr., 日期: 08/20/2023, 浏览量: 399, Q&A: 0

    Synapses are specialized structures that enable neuronal communication, which is essential for brain function and development. Alterations in synaptic proteins have been linked to various neurological and neuropsychiatric disorders. Therefore,

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    Fabrication of Microfluidic Devices for Continuously Monitoring Yeast Aging
    研制用于持续监测酵母老化的微流控装置
    作者:  Richard O’Laughlin, Emerald Forrest, Jeff HastyNan Hao, 日期: 08/05/2023, 浏览量: 269, Q&A: 0

    For several decades, aging in Saccharomyces cerevisiae has been studied in hopes of understanding its causes and identifying conserved pathways that also drive aging in multicellular eukaryotes. While the short lifespan and unicellular nature of

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    Development of a Mouse Model of Hematopoietic Loss of Y Chromosome
    小鼠Y染色体造血丢失模型的建立
    作者:  Soichi SanoKenneth Walsh, 日期: 08/05/2023, 浏览量: 290, Q&A: 0

    This protocol describes the generation of chimeric mice in which the Y chromosome is deleted from a proportion of blood cells. This model recapitulates the phenomenon of hematopoietic mosaic loss of Y chromosome (mLOY), which is frequently observed

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    Fluorescent PCR–based Screening Methods for Precise Knock-in of Small DNA Fragments and Point Mutations in Zebrafish
    基于荧光PCR的斑马鱼DNA小片段精确敲除和点突变筛选方法
    作者:  Blake CarringtonRaman Sood, 日期: 08/05/2023, 浏览量: 333, Q&A: 0

    Generation of zebrafish (Danio rerio) models with targeted insertion of epitope tags and point mutations is highly desirable for functional genomics and disease modeling studies. Currently, CRISPR/Cas9-mediated knock-in is the method of choice for

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