分子生物学

Self-Paced Five-Choice Serial Reaction Time-Task for Mouse Behavioral Testing

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Extraction of DNA and RNA from Formalin-fixed Paraffin-embedded Tissue Specimens
福尔马林固定石蜡包埋组织标本中DNA和RNA的提取

作者: Nidhi Shukla, Narmadhaa Siva, Madhu Balaji Sivakumar, Rabia Parveen, Ashwani Kumar Mishra, Avadh Shah, Krishna Mohan Medicherla 和 Prashanth Suravajhala, 日期: 07/20/2021, 浏览量: 1901, Q&A: 1

One of the most common methods for isolating biomolecules from clinical samples is using TRIzol. While many tissues are freshly frozen, a large number of tissues are formalin-fixed paraffin-embedded (FFPE) blocks for pathology and diagnostics.

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Safe DNA-extraction Protocol Suitable for Studying Tree-fungus Interactions
适于研究树-真菌互作的安全DNA提取方法

作者: Susanna Keriö, Eeva Terhonen 和 Jared M. LeBoldus, 日期: 06/05/2020, 浏览量: 5602, Q&A: 0

We present a safe and low-cost method suitable for DNA extraction from mycelium and tree tissue samples. After sample preparation, the extraction takes about 60 min. Method performance was tested by extracting DNA from various tree tissue samples ...

Different Methods of Soil DNA Extraction
土壤DNA提取的不同方法

作者: Asmita Kamble 和 Harinder Singh, 日期: 01/20/2020, 浏览量: 13347, Q&A: 0

Soil is the major reservoir of microbial diversity. Only 1% of microbial diversity can be cultured while 99% is still not culturable. It is necessary to extract DNA from soil in order to explore the 99% microbial diversity, which will be useful to ...

Isolation of PCR-quality Genomic DNA from Soils Impacted with Extra Heavy Crude Oil
受超重原油污染土壤中PCR级基因组DNA的提取

作者: Laynet A. Puentes , Yusibeska L. Ramos, Ysvic A. Inojosa, César E. Rivera 和 Angela De Sisto, 日期: 06/20/2019, 浏览量: 3661, Q&A: 0

For the study of microbial communities in samples of soils impacted with extra heavy crude oil, it is necessary to perform molecular analyses. Due to the difficulty of oil matrix handling, there are very few protocols reported in writing. Also, one ...

Fusarium graminearum Inoculation on Wheat Head
禾谷镰刀菌的麦穗接种

作者: Chanjing Feng, Huiquan Liu 和 Zhe Tang, 日期: 08/05/2018, 浏览量: 7137, Q&A: 0

Fusarium graminearum, the major causal agent of Fusarium head blight (FHB), causes serious wheat yield losses and a threat to human and animal health. The main efforts to combat the disease are the research of pathogenesis ...

Plasmid Extract from Budding Yeast (Saccharomyces cerevisiae)
芽殖酵母（酿酒酵母）质粒提取

作者: Gal Haimovich, 日期: 07/20/2018, 浏览量: 9654, Q&A: 0

Plasmids are widely used tools in yeast research. In many cases, plasmid libraries are used in genetic screens or in yeast two hybrid screens. In such cases, it is necessary to extract plasmids carrying unknown genetic elements from positive clones ...

CTAB法微量提取柑橘基因组DNA
Citrus Genomic DNA Microextraction Using CTAB Method

作者: 徐远涛, 余惠文, 程运江, 徐强和邓秀新, 日期: 07/10/2018, 浏览量: 4064, Q&A: 0

实验原理：利用CTAB (十六烷基三甲基溴化铵) 作为一种阳离子去污剂，在高离子强度的溶液中与蛋白质和多聚糖形成复合物，而在低离子强度溶液中可以沉淀核酸与酸性多聚糖的特性将DNA从柑橘组织样品中分离出来。
实验目的：快速 ...

高纯度柑橘基因组DNA提取与检测
Extraction and Detection of High-purity Genomic DNA from Citrus

作者: 徐远涛, 余惠文, 程运江, 徐强和邓秀新, 日期: 07/10/2018, 浏览量: 2523, Q&A: 0

实验原理：利用CTAB (十六烷基三甲基溴化铵) ...

An Optimized CTAB Method for Genomic DNA Extraction from Freshly-picked Pinnae of Fern, Adiantum capillus-veneris L.
一种从新鲜收取的铁线蕨样品中提取基因组DNA 的优化CTAB法

作者: Yi Shu, Jin Wan-Ting, Yuan Ya-Ning 和 Fang Yu-Han, 日期: 07/05/2018, 浏览量: 15713, Q&A: 0

As the sister clade of seed plants, ferns are significant materials for plant phylogeny research. However, the genomic DNA extraction protocol for fern samples like modified CTAB method still lacks robustness. Here, we found that the amount and ...

水稻叶片高质量DNA抽提
High-quality DNA Extraction from Rice Leaves

作者: 吴昊, 陈太钰, 林拥军和陈浩, 日期: 06/12/2018, 浏览量: 4332, Q&A: 0

实验原理：植物体内的DNA通常在细胞核中以核蛋白的形式存在，利用阳离子去垢剂CTAB (hexadecyltrimethylammonium bromide，十六烷基三甲基溴化铵) ...