生物化学

分类

    Detection of D-glutamate Production from the Dual Function Enzyme, 4-amino-4-deoxychorismate Lyase/D-amino Acid Transaminase, in Mycobacterium smegmatis
    从垢分支杆菌中通过双功能酶,4-氨基-4-脱氧胆固醇裂解酶/D-氨基酸转氨酶、转氨酶产生的D-谷氨酸的检测
    作者:  Helen K. Opel-Reading, Roman MortuzaKurt L. Krause, 日期: 01/05/2019, 浏览量: 4655, Q&A: 0
    D-amino acid transaminase (D-AAT) is able to synthesize both D-glutamate and D-alanine, according to the following reaction: D-alanine + α-ketoglutarate ⇌ D-glutamate + pyruvate. These two D-amino acids are essential components of the peptidoglycan ...
    水稻组织脯氨酸含量的测定
    Measurment of Proline Content in Rice Tissue
    作者:  都浩, 游均熊立仲, 日期: 04/27/2018, 浏览量: 3181, Q&A: 0
    实验原理:水稻在非生物逆境条件下,体内脯氨酸(proline)含量显著增加。可用磺基水杨酸提取水稻组织中脯氨酸,然后用酸性茚三酮加热处理后,溶液即成红色,再用甲苯萃取使色素全部转移至萃取液中。色素的深浅即表示脯氨酸含量的高低。在520 ...
    Bacterial Competition Assay Based on Extracellular D-amino Acid Production
    基于细胞外D-氨基酸生成的细菌竞争测定
    作者:  Laura AlvarezFelipe Cava, 日期: 04/05/2018, 浏览量: 7326, Q&A: 0
    Bacteria live in polymicrobial communities under tough competition. To persist in a specific niche many species produce toxic extracellular effectors as a strategy to interfere with the growth of nearby microbes. One of such effectors are the ...
    水稻种子氨基酸含量的测定
    Determination of Aino Aid Cntent in Rce Gains
    作者:  彭波李一博, 日期: 03/16/2018, 浏览量: 3640, Q&A: 0
    实验原理:氨基酸为两性分子,在强酸性溶液中,氨基酸以阳离子形式存在,可用阳离子交换分离。在强碱性介质中,氨基酸以阴离子形式存在,可用阴离子交换分离。经过前处理的样品液标本从自动进样器进入保护柱进行预分离,然后再进入分离柱分离,并与泵吸入的茚三酮溶液在混合器混合 ...
    Mass Spectrometry-based in vitro Assay to Identify Drugs that Influence Cystine Solubility
    基于质谱法的体外实验鉴定影响胱氨酸溶解度的药物
    作者:  Neelanjan Bose, Tiffany Zee, Pankaj KapahiMarshall L. Stoller, 日期: 07/20/2017, 浏览量: 7158, Q&A: 0
    Cystinuria is a rare genetic disorder characterized by recurrent, painful kidney stones, primarily composed of cystine, the dimer of the amino acid cysteine (Sumorok and Goldfarb, 2013). Using a mouse model of cystinuria, we have recently shown that ...
    Extraction and Quantification of GABA and Glutamate from Cyanobacterium Synechocystis sp. PCC 6803
    从蓝藻集胞藻属PCC 6803提取和量化GABA和谷氨酸盐
    作者:  Simab KanwalAran Incharoensakdi, 日期: 09/20/2016, 浏览量: 10931, Q&A: 0
    GABA (γ-amino butyric acid) is a biologically active four carbon non-protein amino acid found in prokaryotes and eukaryotes. Glutamate is a five carbon α-amino acid which can be converted to GABA catalyzed by the enzyme glutamate decarboxylase. In ...