Purification and enrichment of DNA origami objects
本实验方法提取自研究论文:
Sequence-programmable covalent bonding of designed DNA assemblies
Sci Adv, Aug 17, 2018; DOI: 10.1126/sciadv.aau1157

After the folding reaction, all reaction products were purified using one round of PEG precipitation (63). The resulting pellet was dissolved in folding buffer (5 mM tris, 1 mM EDTA, and 5 mM NaCl) including 5 mM MgCl2. The final volume was chosen to get a monomer concentration of 100 nM. The samples were equilibrated at 30°C and 450 rpm overnight in a shaker incubator (Thermomix comfort from Eppendorf). All procedures were performed as previously described (64).

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



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