Cells on an eight-well Lab-Tek II chamber (Nunc) were fixed with 4% formaldehyde in PBS at RT for 10 min, permeabilized with 0.1% Triton X-100 in PBS, blocked with 1% BSA in PBS at RT for 1 hour, and incubated with primary antibodies at RT for 1 hour. Primary antibodies for FOXO1 (Cell Signaling) and TIE2 (R&D Systems) were used. The cells were then incubated with secondary antibodies (Invitrogen) in the dark at RT for 1 hour and mounted with VECTASHIELD mounting medium with 4′,6-diamidino-2-phenylindole (Vector Labs). Images were taken with a confocal laser scanning microscope (LSM880, Carl Zeiss).

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



Q&A
请登录并在线提交您的问题
您的问题将发布在Bio-101网站上。我们会将您的问题发送给本研究方案的作者和具有相关研究经验的Bio-protocol成员。我们将通过您的Bio-protocol帐户绑定邮箱进行消息通知。