NF-κB–responsive reporter plasmid (pNF-κB Luc) and Renilla luciferase expression plasmid (phRL-TK) were cotransfected with the indicated expression plasmids into HEK293 cells. Twenty-four hours after transfection, cells were subjected to experiments. Luciferase activity was measured using the Dual-Glo Luciferase Assay System (Promega, Madison, WI, USA). Specific NF-κB Luc activity in individual samples was determined by normalizing firefly luciferase activity to Renilla luciferase activity. For all assays, experiments were performed in triplicate.

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