Hemilateral hindlimb neurectomy was conducted by surgically resecting 5- to 6-mm segments of sciatic and femoral nerves, as described previously (15). As a control sham operation for neurectomy, the contralateral sciatic and femoral nerves were surgically exposed and left unresected. Neurectomy side (right or left) was chosen randomly. Operations were performed 1 week (for biochemical and immunohistochemical studies) or 2 weeks (for μCT and bone histomorphometric studies) before mice were sacrificed at the age of 10 weeks, and subjected to analyses.