To assess smoking status–specific cell enrichment for each cluster, logistic regression was performed using the stats R package (R v3.2.0), modeling each cluster assignment as a function of donor smoking status and the number of cells contributed by each donor. For clusters that were associated with smoking status (FDR q < 0.05), but not the number of cells contributed by each donor, the directionality of the regression coefficient was leveraged to assign never or current smoker status.

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