Human embryonic kidney 293T and MDCK I cell lines were purchased from the American Type Culture Collection. Cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and maintained in a 37°C incubator with 5% CO2. PKD1 null (PN) and heterozygous (PH) cells were provided by Y. Cai (Yale University, New Haven, CT) and cultured as described previously (42).

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