GraphPad Prism 7.0 was used for statistical analysis. Results were presented as means ± SEM for at least three independent experiments. Unpaired two-tailed Student’s t test was used to compare two groups of independent samples, and one-way or two-way analysis of variance (ANOVA) with Tukey’s post hoc test was used to compare multiple groups. P < 0.05 was considered statistically significant.

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