BSR-T7 cells were plated in 96-well plates at 2 × 104 cells per well and transfected the day after with 66 ng of pEMC-N, 44 ng of pEMC-(Flag/L), 28 ng of pEMC-P construct (encoding either wt P or the individual P variants), and 90 ng of plasmid encoding for the minigenome, using jetPRIME (Polyplus). Two days after transfection, the firefly luciferase activity was measured using the Nano-Glo Dual-Luciferase Reporter Assay (Promega). The background luciferase activity observed in the absence of active L protein was subtracted from the signal measured in the presence of L, and data obtained from three independent experiments were normalized to each other using the mean signal observed for all combinations tested at the same time (12).

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



Q&A
请登录并在线提交您的问题
您的问题将发布在Bio-101网站上。我们会将您的问题发送给本研究方案的作者和具有相关研究经验的Bio-protocol成员。我们将通过您的Bio-protocol帐户绑定邮箱进行消息通知。