Diffraction data were integrated by XDS (77) and scaled with AIMLESS (78). Phases were obtained by molecular replacement using the crystal structure of MeV wt P MD (PDB code 3ZDO) (19) using MOLREP (79). Data analysis by the STARANISO server (http://staraniso.globalphasing.org/cgi-bin/staraniso.cgi) revealed an anisotropic diffraction with resolution limits of 2.5 Å along the a* and b* axes and 1.9 Å on the c* axis. To take into account this anisotropy, an ellipsoidal resolution limit mask was used and rescaling was performed by STARANISO. Model building was manually done with COOT (80), and refinement was performed using BUSTER (81) and REFMAC (82). PyMOL (PyMOL Molecular Graphics System, version 2.0, Schrödinger, LLC) was used to visualize and draw the models. The quality of the final model was evaluated using MolProbity (http://molprobity.biochem.duke.edu/). The server PDBeFold from EMBL-EBI (http://www.ebi.ac.uk/msd-srv/ssm/ssmstart.html) was used to compute root mean square deviations (RMSDs) between P MD structures. Coiled-coil analysis was carried out using TWISTER (83).

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



Q&A
请登录并在线提交您的问题
您的问题将发布在Bio-101网站上。我们会将您的问题发送给本研究方案的作者和具有相关研究经验的Bio-protocol成员。我们将通过您的Bio-protocol帐户绑定邮箱进行消息通知。