Total RNA was extracted with TRIzol reagent (Invitrogen) or RNeasy Mini Kit (Qiagen, Hilden, Germany). Synthesis of complementary DNA (cDNA) was performed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA). Quantitative real-time polymerase chain reaction (PCR) was performed using Power SYBR Green PCR Master Mix (Applied Biosystems). Primer sequences are available upon request.

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