Genomic DNA was extracted from entire specimens of B. solaris and P. paranygulgus using the DNeasy Blood & Tissue Kit (QIAGEN), following the manufacturer’s instructions. An 862–base pair (bp) region of the plastid rbcL gene, which encodes for the large subunit of the ribulose-1,5-biphosphate carboxylase/oxygenase, was PCR-amplified using Illustra PuReTaq Ready-To-Go PCR beads (GE Healthcare), and the primers and thermocycling conditions listed in table S1. Amplicons were visualized on 1.5% agarose gels stained with GelRed (Biotium) and enzymatically cleaned before sequencing with Illustra ExoProStar S (GE Healthcare). Clean amplicons were sequenced in 10 μl containing 1 μl of BigDye Terminator (BDT) v3.1 (Applied Biosystems), 2 μl of BDT buffer, 0.5 μM amplification primer, and 1 to 2 μl of PCR product. Cleaned sequencing products were run on an Applied Biosystems 3730S 48-capillary DNA analyzer by the Nucleic Acid Protein Service Unit at UBC. Resulting clean trace files were assembled into full sequences in Geneious v9.1.5 (32) and subjected to a BLAST (Basic Local Alignment Search Tool) search on the National Center for Biotechnology Information (NCBI) website (www.ncbi.nlm.nih.gov) and an identification request in the Public Record Barcode Database on the BOLD (Barcode Of Life Data) website (www.boldsystems.org/index.php/) to verify the plastid’s taxonomic origin.

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