A rheometer (Anton Paar DSR 301) was mounted on top of an inverted confocal microscope (Zeiss Axiovert 200M). We glued a sphere to the rheometer plate and made contact with the fluorescent liquid. The rheometer measures the normal force on the contact. The fluorescent liquid was excited by an argon ion continuous wave laser at 514 nm. The objective lens is a 63× 1.3 numerical aperture LD A-Plan (Zeiss). The filter sets used for filtering of excitation and emission light are dichroic mirror HFT 405/514 and emission filter BP 560|615. Imaging was performed using the Zeiss LSM 5 LIVE microscope control system.

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



Q&A
请登录并在线提交您的问题
您的问题将发布在Bio-101网站上。我们会将您的问题发送给本研究方案的作者和具有相关研究经验的Bio-protocol成员。我们将通过您的Bio-protocol帐户绑定邮箱进行消息通知。