Activity of hOCs in single culture or cocultured with scaffolds with and without hMSCs was evaluated for resorption pit formation on Osteo Assay Microplates. At the completion of the culture period, culture medium was aspirated, and 500 μl of 10% bleach solution was added for 5 min at room temperature. The wells were washed with distilled water and allowed to dry at room temperature for 3 to 5 hours. Pits were observed using a standard microscope and digitally photographed. The percentage of resorption for the whole well of the culture at magnification 2× was calculated by ImageJ.

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