The mouse melanoma cell line B16F10-Luc and the mouse mammary carcinoma cell line 4T1-Luc were obtained from the American Type Culture Collection. B16F10-Luc and 4T1-Luc cells were obtained from PerkinElmer. The culture medium for B16F10-Luc cells was Dulbecco’s modified Eagle’s medium (HyClone) containing 10% fetal bovine serum (Invitrogen) and penicillin and streptomycin (100 U ml−1) (Invitrogen). The culture medium for 4T1-Luc cells was RPMI 1640 medium (HyClone) containing 10% fetal bovine serum (Invitrogen) and penicillin and streptomycin (100 U ml−1) (Invitrogen). Cells were tested every 3 months for potential mycoplasma. Re-authentication of cells was not performed after receipt.

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