The parental breast cancer cell line MDA-MB-231 (42) and derived SCCs were maintained in high-glucose (4.5 mg/ml) Dulbecco’s minimum essential medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin. Cells were maintained at 37°C in a 5% CO2, 95% air incubator.

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