PdBT synthesis
Preparation of azide-presenting biomolecules
Click functionalization of PdBT
1H NMR spectroscopy
GPC determination of molecular mass
Biofunctionalized hydrogel fabrication
Assessment of hydrogel cross-linking by postformation swelling
Differential scanning calorimetry of cross-linking kinetics
Colorimetric assessment of biomolecule incorporation in hydrogels
Assessment of biofunctionalized hydrogel degradation
Leachable cytotoxicity assay
MSC harvest and culture
In vitro MSC encapsulation
Statistical analysis
PEG of manufacturer-reported Mn of 1000 Da, glycolide, triethylamine (TEA), mesyl chloride, CS A sodium salt from bovine trachea, EDC hydrochloride, NHS, 2-(N-morpholino)ethanesulfonic acid (MES), 11-azido-3,6,9-trioxaundecan-1-amine (ATA), 2-azidoacetic acid, Cp*Ru(cod)Cl, DTT, and sodium nitrate were purchased from Sigma-Aldrich (St. Louis, MO) and used as received. High-performance liquid chromatography (HPLC)–grade tetrahydrofuran (THF), dichloromethane (DCM), and methanol were also purchased from Sigma-Aldrich and used as is. Ultrapure water was obtained from a Millipore Super-Q water system (Billerica, MA). PBS was prepared using preallocated powder from Sigma-Aldrich and ultrapure water.