Materials
PdBT synthesis
Preparation of azide-presenting biomolecules
Click functionalization of PdBT
GPC determination of molecular mass
Biofunctionalized hydrogel fabrication
Assessment of hydrogel cross-linking by postformation swelling
Differential scanning calorimetry of cross-linking kinetics
Colorimetric assessment of biomolecule incorporation in hydrogels
Assessment of biofunctionalized hydrogel degradation
Leachable cytotoxicity assay
MSC harvest and culture
In vitro MSC encapsulation
Statistical analysis
NMR was performed using a 600-MHz Bruker spectrometer (Billerica, MA). CDCl3 with 1% (v/v) trimethylsilane and D2O with 1% (w/w) trimethylsilylpropanoic acid from Sigma-Aldrich (St. Louis, MO) were used as NMR solvents. Samples were dissolved at 5 mg/ml in CDCL3 or in D2O in the case of peptide and GAG (glycosaminoglycan) samples, and spectra were processed using Bruker TopSpin software.