Vero E6 cell monolayers were transfected in a 96-well Corning cyclic olefin copolymer polymer plate with the indicated plasmids using FuGENE 6. Transfected cells were incubated for 72 hours in a 37°C incubator with 5% CO2. Cells were rinsed with PBS and fixed in 3.7% formalin for 10 min at room temperature. Fixed wells were subsequently rinsed with PBS and blocked with 7.5% bovine serum albumin (Sigma-Aldrich) in PBS (blocking buffer) overnight at 4°C. Samples were then incubated with mAb-10E11 (5 μg/μl) diluted 1:200 in blocking buffer overnight at 4°C and then rinsed three times with PBS. Following primary antibody incubation, PBS-washed sections were incubated with Alexa Fluor 488–conjugated goat anti-mouse IgG antibody (Invitrogen) diluted (1:1000) in blocking buffer for 1 hour at room temperature. Samples were then incubated with 4′,6-diamidino-2-phenylindole–PBS for 10 min at room temperature, rinsed in PBS, and imaged.

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