Anti–M-segment rabbit sera were produced by DNA vaccination of rabbits using a disposable syringe jet injection device (PharmaJet), as previously described (41). Rabbits were vaccinated with the full-length M segment (pWRG7077/CCHFV–M segmentopt IbAr 10200) at a concentration of 1 mg per dose of plasmid diluted in PBS in a total volume of 0.5 ml per injection. Rabbits were vaccinated three times at 3-week intervals. Four weeks after the final vaccination, sera were collected.

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