Viruses and cells
Anti-CCHFV and isotype antibodies
Mice
Passive protection experiments
Histology
In situ hybridization
Confocal microscopy
Microscopy
Cloning
GP38 purification
Flow cytometry
GP38 capture ELISA
GP38his ELISA
Western blot
VLP production
Immunogold staining and electron microscopy of VLPs
Antibody competition
Statistical analysis
Anti–M-segment rabbit sera were produced by DNA vaccination of rabbits using a disposable syringe jet injection device (PharmaJet), as previously described (41). Rabbits were vaccinated with the full-length M segment (pWRG7077/CCHFV–M segmentopt IbAr 10200) at a concentration of 1 mg per dose of plasmid diluted in PBS in a total volume of 0.5 ml per injection. Rabbits were vaccinated three times at 3-week intervals. Four weeks after the final vaccination, sera were collected.