Human and murine MΦs were prepared and transfected as previously reported (10). For the luciferase assay of 3′UTR vectors of vehicle control and FGL2 (catalog nos. m012 and MT-h07957, Applied Biological Materials Inc.), miR-466l or mock (catalog nos. mm40846 and m003, Applied Biological Materials Inc.) was cotransfected. The luciferase activity was assayed using SuperLight Luciferase reporter assay and measured using a microplate reader (Varioskan Flash, Thermo Fisher Scientific, Shanghai).

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



Q&A
请登录并在线提交您的问题
您的问题将发布在Bio-101网站上。我们会将您的问题发送给本研究方案的作者和具有相关研究经验的Bio-protocol成员。我们将通过您的Bio-protocol帐户绑定邮箱进行消息通知。