Quantitative polymerase chain reaction (PCR) was performed using the SYBR Green System according to the manufacturer’s protocol (Applied Biosystems, Foster City, CA). The expressions of target genes were analyzed using the ΔCt method. The primers for miR-466l (MQP-0101) and 5S ribosomal RNA (rRNA) (MQP-0301) were obtained from Guangzhou RiboBio Co. Ltd. (Guangdong, China). The forward and reverse primers for other genes are as follows:

mFGL2-F GGTGCTCAAAGAAGTGCGGA and mFGL2-R GTTCCTGGACTCTACTGTCCTC; m5-LOX-F CCTATTCCTCCCTGTGTTTCC and m5-LOX-R CACGAGCAGTCCATCATCAC; m12/15-LOX-F GGCTCCAACAACGAGGTCTAC and m12/15-LOX-R CCCAAGGTATTCTGACACATCC; mCOX-2F GGTGCCTGGTCTGATGATGT and mCOX-2-R TGCTGGTTTGGAATAGTTGCT; mLTA4H-F GAGGTCGCGGATACTTGCTC and mLTA4H-R CTCCTGTGACTGGACCGTG; mPGDH-F GGACACACCCATCCTTGAAT and mPGDH-R TCGATGCCGTGATCTTCATA; mβ-ACTIN-F TGACAGGATGCAGAAGGAGA and mβ-ACTIN-R GTACTTGCGCTCAGGAGGAG; hADAM10-F CAACCTACGAATGAAGAGGGACAC and hADAM10-R CCACCACGAGTCTGGATGAATC; hADAM17-F GAAGTGCCAGGAGGCGATTA and hADAM17-R CGGGCACTCACTGCTATTACC; and hβ-ACTIN-F GCGCGGCTACAGCTTCA and hβ-ACTIN-R CTTAATGTCACGCACGATTTCC.

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



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