Experimental materials and instruments
Synthesis of isothiocyanate-benzensulfonamide
Synthesis of N-pep
Synthesis of pepABS
2-Naphthaleneacetic acid-(d)-Phe-(d)-Phe-(d)-Lys-(benzenesulfonamide)-OH
General procedures for hydrogel preparation
TEM sample preparation
CD spectrum
Analysis of intra-/extracellular accumulations of N-pepABS nanofibers
CCK-8 assay
Western blots
Immunofluorescence
Transwell assay
Transfections
SEM images for treated cancer cells
TEM images for treated cancer cells
Flow cytometry
Real-time polymerase chain reaction
Animal experiments
Statistical analysis
MDA-MB-231 cells were cultured with Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin in a CO2 incubator at 37°C. For hypoxia experiments, MDA-MB-231 cells were cultured at 37°C in a hypoxic incubator with 1% O2 and 5% CO2. Before being treated with N-pepABS, MDA-MB-231 cells were precultured in the hypoxic incubator for about 12 hours.