MDA-MB-231 cells were cultured with Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin in a CO2 incubator at 37°C. For hypoxia experiments, MDA-MB-231 cells were cultured at 37°C in a hypoxic incubator with 1% O2 and 5% CO2. Before being treated with N-pepABS, MDA-MB-231 cells were precultured in the hypoxic incubator for about 12 hours.

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