Experimental materials and instruments
Synthesis of isothiocyanate-benzensulfonamide
Synthesis of N-pep
Synthesis of pepABS
2-Naphthaleneacetic acid-(d)-Phe-(d)-Phe-(d)-Lys-(benzenesulfonamide)-OH
General procedures for hydrogel preparation
TEM sample preparation
CD spectrum
Analysis of intra-/extracellular accumulations of N-pepABS nanofibers
Cell cultures
CCK-8 assay
Western blots
Transwell assay
Transfections
SEM images for treated cancer cells
TEM images for treated cancer cells
Flow cytometry
Real-time polymerase chain reaction
Animal experiments
Statistical analysis
Cells were grown on the surface of glasses and treated with drug materials for the indicated time. Before incubation with primary antibodies at 4°C overnight, cells were fixed with 4% paraformaldehyde, treated with 0.1% Triton X-100, and blocked with 2% bovine serum albumin (BSA). After being washed with phosphate-buffered saline (PBS), each sample was incubated with secondary antibodies at room temperature for about 2 hours. After that, each sample underwent a second round of washes and was treated with 4′,6-diamidino-2-phenylindole staining buffer for about 5 min. Last, slides were washed and mounted with the antifade medium immediately.