Experimental materials and instruments
Synthesis of isothiocyanate-benzensulfonamide
Synthesis of N-pep
Synthesis of pepABS
2-Naphthaleneacetic acid-(d)-Phe-(d)-Phe-(d)-Lys-(benzenesulfonamide)-OH
General procedures for hydrogel preparation
TEM sample preparation
CD spectrum
Analysis of intra-/extracellular accumulations of N-pepABS nanofibers
Cell cultures
CCK-8 assay
Western blots
Immunofluorescence
Transwell assay
Transfections
SEM images for treated cancer cells
TEM images for treated cancer cells
Flow cytometry
Animal experiments
Statistical analysis
Total mRNA was extracted with TRIzol agent (Invitrogen) according to the manufacturer’s instructions. Equal amount of RNA was reverse-transcribed to complementary DNA (cDNA) through mixing with Moloney Murine Leukemia Virus reverse transcriptase, deoxynucleoside triphosphate (dNTP), and oligo(dT) according to the protocol from the manufacturer. After that, polymerase chain reaction process was carried out through mixing primers, cDNA, dNTP, and DNA polymerase, while the primers were synthetized by Sangon.