Total mRNA was extracted with TRIzol agent (Invitrogen) according to the manufacturer’s instructions. Equal amount of RNA was reverse-transcribed to complementary DNA (cDNA) through mixing with Moloney Murine Leukemia Virus reverse transcriptase, deoxynucleoside triphosphate (dNTP), and oligo(dT) according to the protocol from the manufacturer. After that, polymerase chain reaction process was carried out through mixing primers, cDNA, dNTP, and DNA polymerase, while the primers were synthetized by Sangon.

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