GraphPad Prism 7 software (GraphPad Software Inc., California, USA) was used for all statistical analysis. For the APC maturation results, mean fluorescence intensity was recorded. Two-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test was applied during the statistical analysis, with P < 0.05 considered statistically significant. The titer of the J8-specific IgG or IgA was described as the lowest dilution that offered an absorbance of greater than 3 SD above the mean absorbance of the negative control wells (wells coated with serum from mice immunized with PBS). A one-way ANOVA followed by Tukey’s post hoc test was applied to statistical analysis of the antibody titers and ELISpot, with P < 0.05 considered statistically significant. The opsonic activity of the antibody (anti-peptide) sera (% reduction in mean CFU) was calculated as [1 − (CFU in the presence of anti-peptide sera)/(mean CFU in the presence of PBS)] × 100. One-way ANOVA followed by Tukey’s post hoc test was applied for the opsonization statistical analysis, with P < 0.05 considered statistically significant. The bacteria infection challenge evaluation of CFU was recorded and analyzed by one-way ANOVA followed by Tukey’s post hoc test, with P < 0.05 considered statistically significant.

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