Crystallographic data collection of CysLT1R-pran crystals was performed at the ID30b beamline of the European Synchrotron Radiation Facility (ESRF), Grenoble, France. Overall, nine CysLT1R-pran datasets (two 90° and seven 10° wedges) were collected from four needle-shaped crystals using a PILATUS 6M detector and an unattenuated 20-μm beam (wavelength, 0.97717 Å). Data were collected using 0.1° to 0.2° oscillations per frame with an exposure calculated by BEST (39), so that the overall dose per dataset was 20 MGy. Data were processed using XDS (x-ray detector software) and scaled with XSCALE (40). Rejection of outliers was performed using correlation coefficients produced by XSCALE, as described (41). Drop of Rmeas in the lowest-resolution shell after an individual dataset exclusion was used as a supporting criterion of its nonisomorphism.

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