hPSCs or ITGA5 KD hPSCs were seeded in a 96-well plate at a density of 2.5 × 103 cells per well. The next day, cells were starved and activated either with or without TGF-β. After 24 hours, cells were washed with PBS and fixed with 4% formaldehyde (Sigma) for 15 min at room temperature. Later, fixed cells were blocked in serum-free medium with 1% BSA for 30 min at room temperature. The cells were incubated with 10 μM AV3-FAM in serum-free medium with 0.1% BSA for 60 min at room temperature. After incubation, the cells were washed with PBS, and nuclei were stained with DAPI followed by imaging using EVOS fluorescence microscope (Thermo Fisher Scientific).

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



Q&A
请登录并在线提交您的问题
您的问题将发布在Bio-101网站上。我们会将您的问题发送给本研究方案的作者和具有相关研究经验的Bio-protocol成员。我们将通过您的Bio-protocol帐户绑定邮箱进行消息通知。