All the animal experiments in this study were performed according to the Institutional Animal Care and Use Committees guidelines and approved by the animal ethical committee of Utrecht University (2014.III.02.022), The Netherlands, and Central Animal Welfare (AVD1100020174305). Collection of patient tissue material was approved by the IRB of Amsterdam Medical Centre (BTC 2014_181) and performed according to the Helsinki Convention guidelines. Informed consent was obtained for all inclusions. Grafting of immunodeficient NSG mice with patient material was performed according to procedures approved by the animal experiment ethical committee (DTB102348/LEX268).