Plasmids construction
Protein expression, purification, and tyrosinase modification
Preparation of LLPS-induced liquid-like droplets and self-assembled amyloid nanofibers
Important parameters that influence the formation of TLC-M liquid-like droplets
Preparation of LLPS-induced DOPA-modified TLC-M nanofiber coatings
CR stain and nitro blue tetrazolium stain assay
ThT assay
Turbidity measurement
Quartz crystal microbalance with dissipation
BCA assay
Underwater adhesion force measurements
Synthesis of CdSeS@ZnS QDs
Adsorption of QDs onto adhesive coatings
Application demonstration of damage repairs
Contact angle measurements
X-ray fiber diffraction of amyloid nanofibers
AFM imaging
SEM imaging
DIC microscopy and confocal fluorescence microscopy imaging
X-ray photoelectron spectroscopy
Statistical analysis
Detailed procedures for sample preparation for TEM imaging are described below: 10 μl of protein solution containing liquid-like droplets or nanofibers was directly deposited on a piece of carbon film–supported 400-mesh copper grid (Beijing Zhongjingkeyi Technology Co. Ltd.) for 10 min. Excessive solution was then quickly wicked away with pieces of filter paper and washed three times with 10 μl of deionized water. Samples were negatively stained with 10 μl of 2 wt % uranyl acetate right away and then dried under an infrared lamp for 20 min. All TEM images were collected on a Tecnai G2 F20 TEM operated at a 120-kV accelerating voltage. Data are presented in Fig. 2B and fig. S2 (B and C).