Plasmids construction
Protein expression, purification, and tyrosinase modification
Preparation of LLPS-induced liquid-like droplets and self-assembled amyloid nanofibers
Important parameters that influence the formation of TLC-M liquid-like droplets
Preparation of LLPS-induced DOPA-modified TLC-M nanofiber coatings
CR stain and nitro blue tetrazolium stain assay
ThT assay
Turbidity measurement
Quartz crystal microbalance with dissipation
BCA assay
Underwater adhesion force measurements
Synthesis of CdSeS@ZnS QDs
Adsorption of QDs onto adhesive coatings
Application demonstration of damage repairs
Contact angle measurements
X-ray fiber diffraction of amyloid nanofibers
TEM imaging
AFM imaging
DIC microscopy and confocal fluorescence microscopy imaging
X-ray photoelectron spectroscopy
Statistical analysis
For sample preparation for SEM analysis, the protein coatings formed on a Teflon plate were washed with deionized water and dried under constant flow of nitrogen gas. To image the interface formed between protein coatings and Teflon plate, the sample was cut vertically using a blade. The cross section of the sample was then imaged using SEM. SEM images were taken using a JSM 7800 SEM operated at a 5-kV accelerating voltage. Data are presented in Fig. 4 (B and F) and fig. S8A.