Plasmids construction
Protein expression, purification, and tyrosinase modification
Preparation of LLPS-induced liquid-like droplets and self-assembled amyloid nanofibers
Important parameters that influence the formation of TLC-M liquid-like droplets
Preparation of LLPS-induced DOPA-modified TLC-M nanofiber coatings
CR stain and nitro blue tetrazolium stain assay
ThT assay
Turbidity measurement
Quartz crystal microbalance with dissipation
BCA assay
Underwater adhesion force measurements
Synthesis of CdSeS@ZnS QDs
Adsorption of QDs onto adhesive coatings
Application demonstration of damage repairs
Contact angle measurements
X-ray fiber diffraction of amyloid nanofibers
TEM imaging
AFM imaging
SEM imaging
X-ray photoelectron spectroscopy
Statistical analysis
Five microliters of protein solution containing liquid-like droplets or nanofibers was dropped on a piece of clean glass slide (1.0 ± 0.2 mm in thickness; Citoglas) and then covered by a piece of cover glass (0.13 to 0.16 mm; microscopic cover glass, Citoglas). DIC imaging was performed on a ZEISS Z2 microscope equipped with Axiocam 506 camera (ZEISS). The 3D confocal images of microfluidic channel or microtubule were obtained with a Leica TCS SP8 confocal microscope. The 561-nm laser was used for excitation of QDs. Data are presented in Figs. 2F and 4 (C and D).