MNFs were purified as described by the Krishma Halai laboratory. Briefly, skin was isolated from newborns at day 1, incubated in Krebs-Ringer Hepes (KRH) buffer containing 10 mM Hepes and 1% penicillin/streptomycin and minced in pieces with a scalpel before digestion in KRH/ATB buffer supplemented with collagenase (1 mg/ml) at 37°C. After centrifugation at 1000 rpm for 5 min, the supernatant was discarded, and the isolated cells put in culture in DMEM, 20% FBS, and 1% penicillin/streptomycin.

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